thrombomiR®
microRNA biomarkers of platelet function
Circulating platelet microRNAs (thrombomiRs®) are novel biomarkers for intrinsic and on-treatment platelet reactivity. thrombomiRs® are secreted from platelets during their activation and can be detected in plasma and serum. Commonly used platelet function tests e.g. LTA, VerifyNow® or PFA-100®, require fresh blood and agonists. Therefore, they clearly lack standardization and their analytical variability may induce misinterpretations. The thrombomiR® kit enables an in vivo measure of platelet function, independently of the activation pathway.
benefits
A one-stop-shop solution to quickly start platelet microRNA biomarker research.
Each kit contains all quality controls required to perform high-quality microRNA biomarker analyses.
1 kit is sufficient for the analysis of 10 distinct platelet microRNAs, 3 endogenous control microRNAs, and 3 synthetic controls in 48 plasma samples.
Cross-species application is possible due to the high sequence conservation of platelet microRNAs.
Fast and simple data analysis using the thrombomiR analysis toolkit
assay requirements
Frozen platelet poor plasma (PPP) aliquots of at least 200 µl
qPCR instrument (read the manual to find out which instruments are supported)
product description
- High throughput: One kit is sufficient for the analysis of 48 platelet poor plasma (PPP) samples
- Low sample volume: 200 µL PPP are recommended (lower volumes can be used but require pre-testing), (-70°C or lower) PPP samples can be used (stability for several years has been demonstrated)
- Reduced hands-on time: primer coated 96 or 384 well plates
- 10 thrombomiR®s and 6 controls/sample are measured per sample
- Fast and simple data analysis: the web-based thrombomiR® software application enables easy data normalization and quality control
For further information about the components included can be found here
Software application
The thrombomiR® analysis toolkit enables
- standardized analysis of qPCR data
- import: upload of raw data files from qPCR instrument and automatic Cq-value calling
- quality control: inspect amplification curves, melting temperature, spike-in and hemolysis controls
- normalization: correction of technical variance and optional imputation of missing values
- export: export normalized Cq-values of all samples and combine automatically with clinical parameters (i.e. age, gender, etc.)
The thrombomiR® analysis toolkit and further information will be made available to all registered customers of the thrombomiR® kit.
intended use
monitor the drug effects on platelet function, reactivity and hemostasis
diagnosis of platelet-related disorders
gain insights into the molecular function of platelets in vivo
determine the effect of a treatment on platelet reactivity in vivo
study the utility of platelet microRNAs as risk factors for cardiovascular and metabolic diseases
price list
| product number | product | size | PCR Cycler compatibility* | price | product Information |
|---|---|---|---|---|---|
| KT-021-TR 96A | thrombomiR® 96-well |
48 samples 16 miRs/sample |
ABI, Bio-Rad, Eppendorf, Stratagene, Takara |
price on request |
product sheet / manual |
| KT-021-TR 96C | thrombomiR® 96-well |
48 samples 16 miRs/sample |
ABI (fast block) | price on request | product sheet / manual |
| KT-021-TR 96D | thrombomiR® 96-well |
48 samples 16 miRs/sample |
Bio-Rad, Stratagene, DNA Engine | price on request | product sheet / manual |
| KT-21-TR 96F | thrombomiR® 96-well |
48 samples 16 miRs/sample |
Roche LC480 I and II | price on request | product sheet / manual |
| KT-021-TR 384E | thrombomiR® 384-well |
48 samples 16 miRs/sample |
ABI, Bio-Rad CFX 384™ | price on request | product sheet / manual |
| KT-021-TR 384G | thrombomiR® 384-well |
48 samples 16 miRs/sample |
Roche LC480 I and II | price on request | product sheet / manual |
* for detailed information on supported cyclers please click here
